Blockveranstaltung: Super-resolution microscopy with DNA-PAINT and dSTORM. Super-resolution fluorescence lifetime imaging microscopy (SR-FLIM) - Details

Blockveranstaltung: Super-resolution microscopy with DNA-PAINT and dSTORM. Super-resolution fluorescence lifetime imaging microscopy (SR-FLIM) - Details

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General information

Course name Blockveranstaltung: Super-resolution microscopy with DNA-PAINT and dSTORM. Super-resolution fluorescence lifetime imaging microscopy (SR-FLIM)
Subtitle
Course number 340406
Semester SoSe 2024
Current number of participants 9
maximum number of participants 10
notwendige Teilnehmeranzahl 2
Home institute Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
Courses type Blockveranstaltung in category Teaching
First date Thursday, 30.05.2024 09:30 - 17:00
Type/Form
Miscellanea First day of the course will take place online via ZOOM, second day in person in lab F02.135, Third Institute of Physics, Faculty of Physics, Friedrich-Hund-Platz 1, 37077 Göttingen.

Exact dates are tba, but the course will take place in June 2024.

Rooms and times

No room preference
Thursday, 30.05.2024 09:30 - 17:00
Friday, 31.05.2024 09:30 - 17:00

Fields of study

Comment/Description

In this course, basics of fluorescence microscopy and super-resolution microscopy techniques will be covered, with the focus on single-molecule localization microscopy techniques. Two of the most important techniques, dSTORM and DNA-PAINT, will be discussed in detail. While dSTORM is more established and easy to implement, DNA-PAINT is a rapidly developing and versatile technique, capable of reaching superior resolution of single nanometers. Recent developments in cellular DNA-PAINT enable to exploit the full potential of DNA-PAINT for multiplexed imaging in fixed cells, then contributing to a deeper understanding of cellular organization and interplay. Advantages, limitations and experimental parameters optimization of dSTORM and DNA-PAINT will be discussed. In the practical part of the course, we will have a hands-on the multiplexed DNA-PAINT imaging of fixed cells.

Course duration: two days. The course will include lecture/seminar/detailed overview of the experimental setups/hands-on session/data analysis training. Following topics will be included:

* Introduction to optical microscopy and super-resolution techniques
* dSTORM imaging
* DNA-PAINT imaging (including multiplexing and variants of DNA-PAINT)
* Microfluidics technology employed for multiplexed Exchange DNA-PAINT imaging
* Multiplexed super-resolution imaging of fixed cells with 10 nm resolution
* Fluorescence lifetime single-molecule localization microscopy
* data analysis of the collected data using different software packages
* quantitative resolution estimation of the obtained super-resolution images



Important:

Once participation has become binding after the signing-out period, cancellation is only possible via the GGNB or GAUSS Office with a) sick certificate, or b) statement by supervisor for scientific reasons. If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support. No exceptions.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates - the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position; (6) that you only receive credits for attending the entire workshop - you will not get partial credits for part-time attendance.

Admission settings

The course is part of admission "GGNB_Methods_2024_SoSe_April_Juni".
You can register and sign out for methods courses in June 2024 from 1-20 April 2024. The slots will be assigned around 25 April 2024.
In exceptional circumstances, you can still sign out until 30 April 2024.
Your participation becomes binding on 2 May 2024. Thereafter, cancellation is only possible via the GGNB Office with a) sick certificate, or b) statement by supervisor (for scientific reasons). If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates – the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation.
The following rules apply for the admission:
  • At least one of these conditions must be fulfilled for enrolment:
    • Subject is Computer Science -PCS-
    • Subject is Molekulare Biologie
    • Subject is PhysicsBiologComplexSyste
    • Subject is Systems Neuroscience
    • Subject is Materialforschung Holz
    • Subject is MolecularBiology of Cells
    • Subject is Geographie
    • Subject is Environmental Informatics
    • Subject is Behavior and Cognition
    • Subject is Theoret Computat Neurosci
    • Subject is Neurowissenschaften
    • Subject is IRTG 2172 - PRoTECT
    • Subject is Psychologie
    • Subject is Molekulare Medizin
    • Subject is Umweltinformatik
    • Subject is Emerging Infect. Diseases
    • Subject is Genome Science
    • Subject is Environmental Informatics
    • Subject is Geoscience
    • Subject is Biologie
    • Subject is Genes in Development, Disease and Evolution
    • Subject is Biologische Diversität und Ökologie
    • Subject is Physik
    • Subject is Microbiology-Biochemistry
    • Subject is Mathematical Sciences
    • Subject is Geography
    • Subject is Chemie
    • Subject is BioNutz-MolwissBiotechnol
    • Subject is Biomol:Struc-Func-Dynamic
    • Subject is Cardiovascular Science
    • Subject is Catalysis Sust.Synt.CaSuS
    • Subject is SensoryMotor Neuroscience
    • Subject is MolecularPhysi oftheBrain
    • Subject is MetalSites i.Biomolecules
  • At least one of these conditions must be fulfilled for enrolment:
    • Degree is PromProgramm Cotutelle
    • Degree is PromStudiengang Cotutelle
    • Degree is Promotionsprogramm
    • Degree is Promotionsstudiengang
    • Degree is Promotion m.Abschlussprfg
    • Degree is Promotion Cotutelle
  • The enrolment is possible from 01.04.2024, 00:00 to 20.04.2024, 23:59.
  • A defined number of seats will be assigned to these courses.
    The seats in the affected courses have been assigned at 25.04.2024 on 23:59. Additional seats may be available via a wait list.
  • Enrolment is allowed for up to 2 courses of the admission set.
Assignment of courses:

Registration mode

The enrolment is binding, participants cannot unsubscribe themselves.