Blockveranstaltung: Basic methods of transmission electron microscopy - from tissue preparation to 3D reconstruction - Details

Blockveranstaltung: Basic methods of transmission electron microscopy - from tissue preparation to 3D reconstruction - Details

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General information

Course name Blockveranstaltung: Basic methods of transmission electron microscopy - from tissue preparation to 3D reconstruction
Subtitle
Course number 340196
Semester SoSe 2024
Current number of participants 0
maximum number of participants 3
notwendige Teilnehmeranzahl 1
Home institute Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
Courses type Blockveranstaltung in category Teaching
Type/Form
Miscellanea The course will take place in presence (BIN, Von Siebold-Str. 3a, Beginning: Foyer BIN) in June/July 2024 (exact dates tba).

The course is given by Prof. Dr. Carolin Wichmann and Julius Bahr.

Rooms and times

Fields of study

Comment/Description

This course will provide basic knowledge and skills on performing electron microscopy of synapses.

Participants will get insights into:



- sample preparation for electron microscopy (due to time limitations partially theoretically)

- Ultrathin sectioning

- Post-staining

- Image acquisition

- Electron-tomography.



In order to prepare inner hair cell ribbon synapses and biological tissues in general for transmission electron microscopy many steps are necessary. The tissue needs to be fixed/immobilized, stained and dehydrated. Moreover, embedding in specific resins is required, followed by trimming steps and ultrathin sectioning. We use this conventional room temperature embedding method to prepare the organ of Corti and/or the anterior ventral cochlear nucleus (AVCN) for electron microscopy. We are interested in the structure-function correlation of the inner hair cell (IHC) ribbon synapses which play a key-role in the hearing process. After passing the IHC ribbon synapses, the signal is transmitted to the AVCN for further processing. We investigate IHC ribbon synapses as well as synapses of the so-called endbulbs of Held in the AVCN and determine parameters such as the number of vesicles, docked vesicles and the vesicle diameter in wild-type or hearing impaired mice using electron microscopy.


Important:

Once participation has become binding after the signing-out period, cancellation is only possible via the GGNB or GAUSS Office with a) sick certificate, or b) statement by supervisor for scientific reasons. If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support. No exceptions.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates - the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position; (6) that you only receive credits for attending the entire workshop - you will not get partial credits for part-time attendance.

Admission settings

The course is part of admission "GGNB_Methods_2024_SoSe_April_Juni".
You can register and sign out for methods courses in June 2024 from 1-20 April 2024. The slots will be assigned around 25 April 2024.
In exceptional circumstances, you can still sign out until 30 April 2024.
Your participation becomes binding on 2 May 2024. Thereafter, cancellation is only possible via the GGNB Office with a) sick certificate, or b) statement by supervisor (for scientific reasons). If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates – the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation.
The following rules apply for the admission:
  • At least one of these conditions must be fulfilled for enrolment:
    • Subject is Computer Science -PCS-
    • Subject is Molekulare Biologie
    • Subject is PhysicsBiologComplexSyste
    • Subject is Systems Neuroscience
    • Subject is Materialforschung Holz
    • Subject is MolecularBiology of Cells
    • Subject is Geographie
    • Subject is Environmental Informatics
    • Subject is Behavior and Cognition
    • Subject is Theoret Computat Neurosci
    • Subject is Neurowissenschaften
    • Subject is IRTG 2172 - PRoTECT
    • Subject is Psychologie
    • Subject is Molekulare Medizin
    • Subject is Umweltinformatik
    • Subject is Emerging Infect. Diseases
    • Subject is Genome Science
    • Subject is Environmental Informatics
    • Subject is Geoscience
    • Subject is Biologie
    • Subject is Genes in Development, Disease and Evolution
    • Subject is Biologische Diversität und Ökologie
    • Subject is Physik
    • Subject is Microbiology-Biochemistry
    • Subject is Mathematical Sciences
    • Subject is Geography
    • Subject is Chemie
    • Subject is BioNutz-MolwissBiotechnol
    • Subject is Biomol:Struc-Func-Dynamic
    • Subject is Cardiovascular Science
    • Subject is Catalysis Sust.Synt.CaSuS
    • Subject is SensoryMotor Neuroscience
    • Subject is MolecularPhysi oftheBrain
    • Subject is MetalSites i.Biomolecules
  • At least one of these conditions must be fulfilled for enrolment:
    • Degree is PromProgramm Cotutelle
    • Degree is PromStudiengang Cotutelle
    • Degree is Promotionsprogramm
    • Degree is Promotionsstudiengang
    • Degree is Promotion m.Abschlussprfg
    • Degree is Promotion Cotutelle
  • The enrolment is possible from 01.04.2024, 00:00 to 20.04.2024, 23:59.
  • A defined number of seats will be assigned to these courses.
    The seats in the affected courses have been assigned at 25.04.2024 on 23:59. Additional seats may be available via a wait list.
  • Enrolment is allowed for up to 2 courses of the admission set.
Assignment of courses:

Registration mode

The enrolment is binding, participants cannot unsubscribe themselves.