Veranstaltungsdaten

Beschreibung: In this course, basics of fluorescence microscopy and super-resolution microscopy techniques will be covered, with the focus on single-molecule localization microscopy techniques. Two of the most important techniques, dSTORM and DNA-PAINT, will be discussed in detail. While dSTORM is more established and easy to implement, DNA-PAINT is a rapidly developing and versatile technique, capable of reaching superior resolution of single nanometers. Recent developments in cellular DNA-PAINT enable to exploit the full potential of DNA-PAINT for multiplexed imaging in fixed cells, then contributing to a deeper understanding of cellular organization and interplay. Advantages, limitations and experimental parameters optimization of dSTORM and DNA-PAINT will be discussed. In the practical part of the course, we will have a hands-on the multiplexed DNA-PAINT imaging of fixed cells.

Course duration: two days. The course will include lecture/seminar/detailed overview of the experimental setups/hands-on session/data analysis training. Following topics will be included:

* Introduction to optical microscopy and super-resolution techniques
* dSTORM imaging
* DNA-PAINT imaging (including multiplexing and variants of DNA-PAINT)
* Microfluidics technology employed for multiplexed Exchange DNA-PAINT imaging
* Multiplexed super-resolution imaging of fixed cells with 10 nm resolution
* Fluorescence lifetime single-molecule localization microscopy
* data analysis of the collected data using different software packages
* quantitative resolution estimation of the obtained super-resolution images

Important:

Once participation has become binding after the signing-out period, cancellation is only possible via the GGNB or GAUSS Office with a) sick certificate, or b) statement by supervisor for scientific reasons. If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support. No exceptions.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates - the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position; (6) that you only receive credits for attending the entire workshop - you will not get partial credits for part-time attendance.