Vorlesung und Praktikum: M.iPAB.0022: Molecular Genetics and Genomics - Details

Vorlesung und Praktikum: M.iPAB.0022: Molecular Genetics and Genomics - Details

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Course name Vorlesung und Praktikum: M.iPAB.0022: Molecular Genetics and Genomics
Subtitle
Course number 740865
Semester WiSe 2024/25
Current number of participants 0
Home institute Nutzpflanzengenetik
Courses type Vorlesung und Praktikum in category Teaching
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Fields of study

Module assignments

Comment/Description

Contents:
Lecture Contents
• Overview on molecular methods in gene and genome analysis
• Theoretical basis of classical and new marker technologies
• Methodology, areas of use, and automation of sequencing technologies
• Applications in applied breeding and breeding research
Practical Contents
Basics of molecular biology practical work with nucleic acids by means of discrete
performing polymerase chain reactions (PCR), short sequence repeats (SSR) and single
nucleotide polymorphism (SNP) marker protocols.
Robotics for high-throughput and miniaturization of molecular biology methods by means
of using pipetting robots for single steps of the custom procedures.
Custom procedures for genome and transcriptome analysis:
• Production of sequencing libraries for genotyping DNA by sequencing (GBS).
• Production of sequencing libraries for strand specific 3" targeted gene expression
analysis by Digital Gene Expression RNA sequencing (3" DGE RNA-seq).

The students are able to plan and perform complex molecular techniques independently
and to assess their suitability for breeding related questions considering scientific and
economic factors.

Examination requirements:
Regular attendance of practical (minimum of 90%).
Formal protocol with scientifically sound lab work documentation including introduction,
methods, results and discussion.
Knowledge on practical implementation, execution and applicability of molecular marker
and sequencing technology in research and breeding

Admission settings

The course is part of admission "Anmeldung gesperrt (LIZA)".
Eintragen nicht möglich, diese Veranstaltung ist (noch) gesperrt. Bitte lesen Sie unbedingt die Hinweise zur Veranstaltungsanmeldung !
The following rules apply for the admission:
  • Admission locked.