Blockveranstaltung: Nerve cell culture and patch-clamp recordings from nerve cells - Details

Blockveranstaltung: Nerve cell culture and patch-clamp recordings from nerve cells - Details

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Wednesday, 09.10.2024 09:00 - 13:00
Thursday, 10.10.2024 09:00 - 13:00

Fields of study

Comment/Description

To study synaptic transmission mechanisms, my lab takes advantage of the single cell autaptic neuron culture system. This model system is ideally suitable for understanding the most important parameters underlying synaptic communication in a quantitative fashion. It is unique, as all synapses originate from a single axon. Thus different synaptic release modes can be quantified.

Step 1. Preparing autaptic neuron cultures

The autaptic preparation is defined by a single neuron that resides on an island of astrocytes with limited size, called a microisland culture. First, course participants will learn how the micoisland astrocyte culture is made and developed. Second, course participants will learn how to grow single neurons on the astrocyte islands. The applicants will learn to dissociate neurons from target areas of the mouse brain and to culture them on the astrocyte feeder culture.

Step 2. Measuring evoked synaptic transmission in autaptic cultures

In autaptic neuron cultures, all synapses that contact to the dendrite of the neuron are formed by a single axon of the same neuron. Thus, all synapses can be stimulated to release transmitter at once by brief somatic depolarization. To understand the evoked synaptic responses, my lab uses a basic application of the patch clamp technique. Course participants will learn the basics of the measurement and quantification of synaptic responses in autaptic neuron cultures.

This course is intended for students who want to explore projects concerned with synaptic function in neurons.


Important:

Once participation has become binding after the signing-out period, cancellation is only possible via the GGNB or GAUSS Office with a) sick certificate, or b) statement by supervisor for scientific reasons. If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support. No exceptions.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates - the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position; (6) that you only receive credits for attending the entire workshop - you will not get partial credits for part-time attendance.

Admission settings

The course is part of admission "GGNB_Methods_2024_WiSe_August_Oktober".
You can register and sign out for methods courses in October 2024 from 1-20 August 2024. The slots will be assigned around 25 August 2024.
In exceptional circumstances, you can still sign out until 31 August 2024.
Your participation becomes binding on 1 September 2024. Thereafter, cancellation is only possible via the GGNB Office with a) sick certificate, or b) statement by supervisor (for scientific reasons). If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates – the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position; (6) that you only receive credits for attending the entire workshop - you will not get partial credits for part-time attendance.
The following rules apply for the admission:
  • A defined number of seats will be assigned to these courses.
    The seats in the affected courses have been assigned at 25.08.2024 on 23:59. Additional seats may be available via a wait list.
  • Enrolment is allowed for up to 2 courses of the admission set.
  • At least one of these conditions must be fulfilled for enrolment:
    • Degree is Promotion m.Abschlussprfg
    • Degree is PromProgramm Cotutelle
    • Degree is PromStudiengang Cotutelle
    • Degree is Promotionsprogramm
    • Degree is Promotionsstudiengang
    • Degree is Promotion Cotutelle
  • At least one of these conditions must be fulfilled for enrolment:
    • Subject is Behavior and Cognition
    • Subject is Biomol:Struc-Func-Dynamic
    • Subject is MolecularBiology of Cells
    • Subject is Molekulare Biologie
    • Subject is Emerging Infect. Diseases
    • Subject is Biologische Diversität und Ökologie
    • Subject is Molekulare Medizin
    • Subject is Environmental Informatics
    • Subject is BioNutz-MolwissBiotechnol
    • Subject is Systems Neuroscience
    • Subject is Geoscience
    • Subject is Mathematical Sciences
    • Subject is Umweltinformatik
    • Subject is SensoryMotor Neuroscience
    • Subject is IRTG 2172 - PRoTECT
    • Subject is Theoret Computat Neurosci
    • Subject is PhysicsBiologComplexSyste
    • Subject is Geography
    • Subject is Computer Science -PCS-
    • Subject is Materialforschung Holz
    • Subject is Genome Science
    • Subject is Psychologie
    • Subject is MolecularPhysi oftheBrain
    • Subject is Environmental Informatics
    • Subject is Catalysis Sust.Synt.CaSuS
    • Subject is Biologie
    • Subject is Cardiovascular Science
    • Subject is Neurowissenschaften
    • Subject is Physik
    • Subject is Microbiology-Biochemistry
    • Subject is Chemie
    • Subject is Geographie
    • Subject is Genes in Development, Disease and Evolution
    • Subject is MetalSites i.Biomolecules
  • The enrolment is possible from 01.08.2024, 00:00 to 20.08.2024, 23:59.
Assignment of courses:

Registration mode

The enrolment is binding, participants cannot unsubscribe themselves.