General information

Course name	Blockveranstaltung: Investigating macromolecular complexes by density gradient centrifugation
Subtitle
Course number	340415
Semester	WiSe 2024/25
Current number of participants	1
maximum number of participants	5
notwendige Teilnehmeranzahl	1
Home institute	Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
Courses type	Blockveranstaltung in category Teaching
Type/Form
Miscellanea	This two-day course will take place in October 2024 (exact date tba) in presence. It will be given by Prof. Dr. Ricarda Richter-Dennerlein.

Lehrende

Dr. Ricarda Richter-Dennerlein

Rooms and times

Fields of study

Comment/Description

Topic:
Density gradient centrifugation is widely used for separating cellular organelles or macromolecular complexes. During the course the biophysical background of ultracentrifugation will be discussed as well as experimental design (e.g. centrifugation speed, rotors, chemical compounds of gradient solutions) for specific scientific goals. As an example, we will perform sucrose density gradient centrifugation with mitochondrial extracts to separate and analyze the mitochondrial ribosomal subunits.

Schedule:
Day 1:
- Theoretical introduction into sucrose gradient ultracentrifugation and Biocomp gradient station; Discussing the experimental workflow;
- Isolation of mitochondria from cultured human cells;
- Gradient preparation;
- Loading of mitochondrial lysates on a gradient and starting the ultracentrifuge.

Day 2:
- Fractionating the sucrose gradient using Biocomp gradient station;
- Discussing the results.

At the end of the course the participants should be able to plan and perform sucrose gradient ultracentrifugation and adjust experimental parameters for their specific scientific goals.

Important:

Once participation has become binding after the signing-out period, cancellation is only possible via the GGNB or GAUSS Office with a) sick certificate, or b) statement by supervisor for scientific reasons. If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support. No exceptions.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates - the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position; (6) that you only receive credits for attending the entire workshop - you will not get partial credits for part-time attendance.

Admission settings

The course is part of admission "GGNB_Methods_2024_WiSe_August_Oktober".

You can register and sign out for methods courses in October 2024 from 1-20 August 2024. The slots will be assigned around 25 August 2024.
In exceptional circumstances, you can still sign out until 31 August 2024.
Your participation becomes binding on 1 September 2024. Thereafter, cancellation is only possible via the GGNB Office with a) sick certificate, or b) statement by supervisor (for scientific reasons). If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates – the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position; (6) that you only receive credits for attending the entire workshop - you will not get partial credits for part-time attendance.
The following rules apply for the admission:

A defined number of seats will be assigned to these courses.
The seats in the affected courses have been assigned at 25.08.2024 on 23:59. Additional seats may be available via a wait list.
Enrolment is allowed for up to 2 courses of the admission set.
At least one of these conditions must be fulfilled for enrolment:
- Degree is Promotion m.Abschlussprfg
- Degree is PromProgramm Cotutelle
- Degree is PromStudiengang Cotutelle
- Degree is Promotionsprogramm
- Degree is Promotionsstudiengang
- Degree is Promotion Cotutelle
At least one of these conditions must be fulfilled for enrolment:
- Subject is Behavior and Cognition
- Subject is Biomol:Struc-Func-Dynamic
- Subject is MolecularBiology of Cells
- Subject is Molekulare Biologie
- Subject is Emerging Infect. Diseases
- Subject is Biologische Diversität und Ökologie
- Subject is Molekulare Medizin
- Subject is Environmental Informatics
- Subject is BioNutz-MolwissBiotechnol
- Subject is Systems Neuroscience
- Subject is Geoscience
- Subject is Mathematical Sciences
- Subject is Umweltinformatik
- Subject is SensoryMotor Neuroscience
- Subject is IRTG 2172 - PRoTECT
- Subject is Theoret Computat Neurosci
- Subject is PhysicsBiologComplexSyste
- Subject is Geography
- Subject is Computer Science -PCS-
- Subject is Materialforschung Holz
- Subject is Genome Science
- Subject is Psychologie
- Subject is MolecularPhysi oftheBrain
- Subject is Environmental Informatics
- Subject is Catalysis Sust.Synt.CaSuS
- Subject is Biologie
- Subject is Cardiovascular Science
- Subject is Neurowissenschaften
- Subject is Physik
- Subject is Microbiology-Biochemistry
- Subject is Chemie
- Subject is Geographie
- Subject is Genes in Development, Disease and Evolution
- Subject is MetalSites i.Biomolecules
The enrolment is possible from 01.08.2024, 00:00 to 20.08.2024, 23:59.

Assignment of courses:

Registration mode

The enrolment is binding, participants cannot unsubscribe themselves.

Blockveranstaltung: Investigating macromolecular complexes by density gradient centrifugation - Details