Blockveranstaltung: Extended Methods Course: ENI Electrophysiology Training (ENI-ELECTRAIN) - Details

Blockveranstaltung: Extended Methods Course: ENI Electrophysiology Training (ENI-ELECTRAIN) - Details

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General information

Course name Blockveranstaltung: Extended Methods Course: ENI Electrophysiology Training (ENI-ELECTRAIN)
Subtitle
Course number 340095
Semester SoSe 2024
Current number of participants 2
maximum number of participants 8
Home institute Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)
Courses type Blockveranstaltung in category Teaching
First date Monday, 27.05.2024 09:00 - 18:00
Type/Form
Miscellanea Locations:
European Neuroscience Institute (ENI-G), Grisebachstr. 5, 37077 Göttingen
Institute for Auditory Neuroscience, University Medical Center Göttingen (UMG), Robert-Koch-Str. 40, 37075 Göttingen (practical topic 4)

Rooms and times

No room preference
Monday, 27.05.2024 09:00 - 18:00
Tuesday, 28.05.2024 09:00 - 18:00
Wednesday, 29.05.2024 09:00 - 18:00
Thursday, 30.05.2024 09:00 - 18:00
Friday, 31.05.2024 09:00 - 18:00

Fields of study

Comment/Description

ENI-ELECTRAIN 2024



TOPIC 1: Oocyte recordings with recombinant channels

(PARDO)



TOPIC 2:Invertebrate recordings

(FERBER)



TOPIC 3:Slice electrophysiology

(CARTER)



TOPIC 4: Channelrhodopsin photocurrent measurements and optogenetic stimulation of rat hippocampal neurons

(MAGER)



Practical Part

Monday, Tuesday, Thursday and Friday from 13:30 - 18:00h
Wednesday from 9:00 - 13:30h



1. Luis Pardo - ENI teaching lab

Topic: Expression and electrophysiological characterization of different ion-channels in the Xenopus oocyte expression system

Techniques: cDNA expression techniques in Xenopus oocytes, Two-electrode voltage clamp configuration and measurements, Quantitative evaluation and statistical analysis of different ion channels/conductances



1. Michael Ferber - ENI teaching lab

Topic: In-vivo electrophysiology of identified neurons in Hirudo medicinalis

Techniques: Single and double intracellular recording techniques, Characterization of spontaneous and stimulus-evoked electrical activity patterns in identified neurons, Analysis of synaptic connectivity and network properties, Pharmacological characterization of different electrical conductances.



3. Brett Carter - ENI teaching lab

Topic: Measurement of synaptic parameters in mouse hippocampal slices

Techniques: Miniature EPSC recording of CA1 pyramidal cells, evoked AMPA receptor and NMDA receptor mediated synaptic transmission of Schaffer collateral CA1 pyramidal cell synapses, lentiviral-mediated molecular manipulation of CA1 pyramidal cells



4. Thomas Mager - Institute for Auditory Neuroscience (UMG)

Topic: Channelrhodopsin photocurrent measurements and optogenetic stimulation of rat hippocampal neurons

Techniques: Optogenetics, Patch-Clamp technique, Cell culture work, Data analysis, Adeno-associated virus mediated transduction, Photostimulation of rat hippocampal neurons



Presentation of results & Cleaning-up: Friday, ENI Lecture Hall & ENI Teaching Labs



Lectures

Monday - Friday from 9:00h ENI lecture hall

(open to all GGNB students)

Mon, 27th of May 09:00-10:00 Introduction to electrophysiological methods (Pardo)
Mon, 27th of May 10:00-11:00 Heterologous expression and study of ion channels in oocytes (Terlau tbc / Pardo)
Mon, 27th of May 11:00-12:00 Ligand-gates channels (Nicke)


Tue, 28th of May 09:00-10:00 Basic electronics (Intro; Carter/Schliephacke tbc)
Tue, 28th of May 10:00-11:00 Electrophysiological instrumentation (Intro; Carter/Schließhacke tbc)
Tue, 28th of May 11:00-12:00 Introduction to slice electrophysiology (Carter)


Wed, 29th of May 15:00-16:00 Calcium imaging techniques (Milosevic, online)
Wed, 29th of May 16:00-17:30 Lipids, Fusion and Membrane Properties (Woodbury, online)
Wed, 29th of May 17:30-19:00 Data acquisition, noise, and the FFT (Woodbury, online)

Thu, 30th of May 09:00-10:00 Introduction to optogenetic techniques (Mager)
Thu, 30th of May 10:00-11:00 Voltage gated ion channels (Pardo)
Thu, 30th of May 11:00-12:00 Invertebrate model systems in neuroscience (Ferber)

Fri, 31th of May 09:00-10:00 Electrophysiology and imaging methods monitoring hair cell synapse (Pangrsic)
Fri, 31th of May 10:00-11:00 Chronic implants and wireless electrophysiology in freely moving animals (Gail)
Fri, 31th of May 11:00-12:00 fMRI guided in vivo electrophysiology (Schwiedrzik)



Faculty:

Brett CARTER Synaptic Physiology and Plasticity, ENI Göttingen
Michael FERBER XLAB, Göttingen
Alexander GAIL Sensorimotor Transformations, German Primate Center Göttingen
Thomas MAGER Auditory Neuroscience, University Medical Center Göttingen, MBExC
Ira MILOSEVIC Neuronal Physiology and Pathology Group, University of Oxford
Annette NICKE Institute for Pharmacology and Toxicology, University of Munich
Tina PANGRSIC Institute for Auditory Neuroscience, University Medical Center Göttingen
Luis PARDO Molecular Biology of Neuronal Signals, MPI-em Göttingen
Ralph SCHLIEPHACKE, Schliephacke Computerized Infrastructure Solutions, Göttingen tbc
Caspar SCHWIEDRZIK Neural Circuits and Cognition, ENI Göttingen
Heinrich TERLAU Christian Albrechts University, Kiel tbc
Dixon WOODBURY Cell Biology & Physiology, Brigham Young University, USA
Important:

Once participation has become binding after the signing-out period, cancellation is only possible via the GGNB or GAUSS Office with a) sick certificate, or b) statement by supervisor for scientific reasons. If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support. No exceptions.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates - the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation; (5) inform us if you no longer wish to maintain a waiting list position; (6) that you only receive credits for attending the entire workshop - you will not get partial credits for part-time attendance.

Admission settings

The course is part of admission "GGNB_Methods_2024_SoSe_März_Mai".
You can register and sign out for methods courses in May 2024 from 1-20 March 2024. The slots will be assigned around 25 March 2024.
In exceptional circumstances, you can still sign out until 31 March 2024.
Your participation becomes binding on 1 April 2024. Thereafter, cancellation is only possible via the GGNB Office with a) sick certificate, or b) statement by supervisor (for scientific reasons). If you do not provide this, you will be excluded from courses for the next 12 months, as well as travel grants and other financial support.

By signing up you agree (1) to carefully check whether you will be able to attend the course (do not register for 2 courses taking place on the same dates – the above mentioned sanctions apply!), (2) to frequently check your student e-mail account, as all information on courses will exclusively be sent to that account; (3) that your name and e-mail address may be forwarded to the course instructor(s) for communication before and after the course; (4) to complete the course evaluation.
The following rules apply for the admission:
  • The enrolment is possible from 01.03.2024, 00:00 to 20.03.2024, 23:59.
  • Enrolment is allowed for up to 2 courses of the admission set.
  • At least one of these conditions must be fulfilled for enrolment:
    • Field of study is IRTG 2172 - PRoTECT
    • Field of study is Biomol:Struc-Func-Dynamic
    • Field of study is Geographie
    • Field of study is BioNutz-MolwissBiotechnol
    • Field of study is Biologische Diversität und Ökologie
    • Field of study is PhysicsBiologComplexSyste
    • Field of study is Environmental Informatics
    • Field of study is Neurowissenschaften
    • Field of study is Emerging Infect. Diseases
    • Field of study is Molekulare Biologie
    • Field of study is Mathematical Sciences
    • Field of study is MolecularBiology of Cells
    • Field of study is Biologie
    • Field of study is Chemie
    • Field of study is Genome Science
    • Field of study is MetalSites i.Biomolecules
    • Field of study is SensoryMotor Neuroscience
    • Field of study is Geography
    • Field of study is Environmental Informatics
    • Field of study is Cardiovascular Science
    • Field of study is Microbiology-Biochemistry
    • Field of study is Umweltinformatik
    • Field of study is Psychologie
    • Field of study is Systems Neuroscience
    • Field of study is Catalysis Sust.Synt.CaSuS
    • Field of study is Theoret Computat Neurosci
    • Field of study is Materialforschung Holz
    • Field of study is Geoscience
    • Field of study is Physik
    • Field of study is Behavior and Cognition
    • Field of study is Genes in Development, Disease and Evolution
    • Field of study is MolecularPhysi oftheBrain
    • Field of study is Molekulare Medizin
    • Field of study is Computer Science -PCS-
  • A defined number of seats will be assigned to these courses.
    The seats in the affected courses have been assigned at 25.03.2024 on 23:59. Additional seats may be available via a waiting list.
  • At least one of these conditions must be fulfilled for enrolment:
    • Degree is Promotion Cotutelle
    • Degree is PromProgramm Cotutelle
    • Degree is Promotion m.Abschlussprfg
    • Degree is PromStudiengang Cotutelle
    • Degree is Promotionsstudiengang
    • Degree is Promotionsprogramm
Assignment of courses:

Registration mode

The enrolment is binding, participants cannot unsubscribe themselves.